anti integrin β 6  (Proteintech)

Journal: Journal of Biomedicine and Biotechnology

Article Title: Low-Frequency Low-Intensity Ultrasounds Do Not Influence the Survival and Immune Functions of Cultured Keratinocytes and Dendritic Cells

doi: 10.1155/2009/193260

Figure Lengend Snippet: US at 42 KHz and 0.15 W/cm 2 do not alter membrane molecule expression in human keratinocytes. Keratinocytes were analyzed for β 1 and α 6 β 4 integrins, ICAM-1 and HLA-DR expression by flow cytometry after 1–3 cycles of US or treatment with IFN- γ plus TNF- α . Dotted lines represent staining with matched isotype immunoglobulins. The x -axis and the y -axis indicate the relative cell number and fluorescence intensity, respectively.

Article Snippet: Keratinocyte expression of membrane ICAM-1, HLA-DR, and integrins was evaluated using fluorescein isothiocyanate- (FITC-) conjugated anti-CD54 (clone 84H10; Immunotech, Marseille, France), anti-HLA-DR (clone L243, BD Pharmingen, Franklin Lakes, NJ, USA), anti-human α 6 β 4 integrin (clone JB1a, Chemicon, Temecula, CA), and anti-human β 1 integrin (clone S341) monoclonal Abs.

Techniques: Expressing, Flow Cytometry, Staining, Fluorescence

Journal: Journal of Biomedicine and Biotechnology

Article Title: Low-Frequency Low-Intensity Ultrasounds Do Not Influence the Survival and Immune Functions of Cultured Keratinocytes and Dendritic Cells

doi: 10.1155/2009/193260

Figure Lengend Snippet: Effects of low-frequency US at 0.15–7 W/cm 2 intensity range on cultured keratinocytes and DC. Keratinocyte and DC cultures were treated with 42 KHz US at the indicated intensities for 7 minutes (1 cycle). Apoptotic cells were determined by annexin V/PI staining and FACS analysis. Apoptotic cell percentage (%) includes P I + , Ann V/PI + , as well as Ann V + subpopulations. The expression of surface markers was revealed by performing staining with specific Abs and FACS analysis. Membrane molecule positivity is expressed as ΔMFI, which represents the mean fluorescence intensity of samples stained with specific Abs subtracted of the fluorescence of Ig-stained controls. Supernatants from keratinocyte and DC cultures were tested by ELISA to measure chemokine and cytokine contents. Phospho-ERK1/2 were revealed by Western blotting on lysates obtained from US-treated keratinocytes. Phopho-ERK1/2 amounts were calculated by densitometry and are expressed as fold induction (F.I.) in experimental relative to untreated samples, to which were given a value of 1.

Article Snippet: Keratinocyte expression of membrane ICAM-1, HLA-DR, and integrins was evaluated using fluorescein isothiocyanate- (FITC-) conjugated anti-CD54 (clone 84H10; Immunotech, Marseille, France), anti-HLA-DR (clone L243, BD Pharmingen, Franklin Lakes, NJ, USA), anti-human α 6 β 4 integrin (clone JB1a, Chemicon, Temecula, CA), and anti-human β 1 integrin (clone S341) monoclonal Abs.

Techniques: Cell Culture, Staining, Expressing, Fluorescence, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Advanced Science

Article Title: Inhibition of Integrin α v β 6 Activation of TGF‐ β Attenuates Tendinopathy

doi: 10.1002/advs.202104469

Figure Lengend Snippet: Integrin α v β 6 active latent TGF‐ β in response to mechanical stress drives the progression of tendinopathy. A) Representative images and B) quantification of immunostaining of tendon sections with antibodies against α v β 6 at 1, 4, and 8 weeks after DI or sham processing. Scale bar: 50 µm. All data are shown as the mean ± standard deviation ( n = 8 mice per group). * p < 0.05 compared with sham group as determined by one‐way analysis of variance. C) A schematic diagram of the ex vivo tensile model. D) Frequency distribution of tendon fiber orientation. E,G) Representative images and F,H) quantification of E,F) pSmad2 + cells and (G, H) TUNEL + cells. Scale bars: 50 µm. I) Western blot of pSmad2 and Smad2 levels in tendon lysates. J) Quantification of pSmad2 relative density relative to physiological loading group (20 g) in tendon lysates after above‐mentioned treatment. K) Quantitative analysis of maximum tensile force and stiffness of tendons. All data from B, F, H, J, and K are shown as the mean ± standard deviation ( n = 8 per group). * p < 0.05 compared with 200 g loaded group as determined by one‐way analysis of variance.

Article Snippet: Sections were incubated with primary antibodies to human CD68 (Abcam, Cambridge, UK, ab955, 1:200), human Col2 (Abcam, ab34712, 1:100), human MMP13 (Abcam, ab3208, 1:40), human/mouse pSmad2 (Santa Cruz Biotechnology, Dallas, TX, sc‐11769, 1:50), mouse Col2 (Abcam, ab185430, 1:100), mouse CD68 (Abcam, ab125212, 1:100), mouse MMP13 (Abcam, ab219620, 1:100), mouse green fluorescent protein (Abcam, ab290, 1:200), and integrin α v β 6 (Bioss Antibodies, Woburn, MA, bs‐5791R‐Biotin, 1:100) overnight at 4 °C.

Techniques: Immunostaining, Standard Deviation, Ex Vivo, TUNEL Assay, Western Blot